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High-performance liquid chromatographic preparation of galactosyl-hydroxylysine, a specific bone collagen marker. 1989
L Moro, and
C Battista, and
C Modricky, and
L Rovis, and
B De Bernard, and
P Traldi, and
G Lubrano, and
F Marotti
Dipartimento di Biochimica, Universitá degli Studi di Trieste, Italy.
Galactosyl-hydroxylysine, a specific bone collagen marker, has been prepared directly from human urine samples by high-performance liquid chromatography (HPLC) on a preparative column. The compound is the didansyl derivative, as proved by HPLC and mass spectrometry under fast atom bombardment conditions. Since this compound is not commercially available, the procedure reported appears to be the simplest way to prepare it, which is necessary to measure the urinary excretion of this collagen metabolite by HPLC.
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