BIOMAT Database Logo BIOMAT Database Logo

Expression and characterization of the interleukin 2 receptor in Theileria parva-infected bovine lymphocytes. 1989

T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie von Spaltstoffen, FRG.

We have previously shown that interleukin 2 receptors (IL2R) are constitutively expressed on the surface of bovine lymphocytes infected with the parasite Theileria parva (Dobbelaere, D.A.E. et al., Proc. Natl. Acad. Sci. USA 1988. 85: 4730). In the present work we characterized these further and showed that IL2R (Tac antigen) gene expression depended on the continuous presence of the parasite in the host cell cytoplasm. By Northern blot analysis we showed that elimination of the parasite, using a specific theilericidal drug, led to the arrest of Tac antigen mRNA expression. We also investigated receptor internalization and reappearance after receptor-mediated endocytosis. Binding of human recombinant interleukin 2 (hrIL2) to the bovine IL2R caused rapid internalization of the surface IL2/IL2R complex. Approximately 50% of 125I-labeled hrIL2 was internalized within 10 min. The reappearance of surface IL2R after ligand-mediated endocytosis was also studied. Fifty percent of the maximum level of free IL2R reappeared within 1-1.5 h, but approximately 12 h were needed to restore normal levels of free IL2R expression after blocking with excess unlabeled hrIL2.

UI MeSH Term Description Entries
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004705 Endocytosis Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis. Endocytoses
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013801 Theileriasis Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition. Corridor Disease,East Coast Fever,Theileriosis,Coast Fever, East,Disease, Corridor,Fever, East Coast,Theileriases,Theilerioses

Related Publications

T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Expression of Tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with Theileria parva or Theileria annulata.
December 1990, Infection and immunity,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes.
December 1998, Veterinary parasitology,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Constitutive IL-2 mRNA expression in lymphocytes, infected with the intracellular parasite Theileria parva.
July 1992, Journal of immunology (Baltimore, Md. : 1950),
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Theileria parva in cattle: characterization of infected lymphocytes and the immune responses they provoke.
February 1989, Veterinary immunology and immunopathology,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
The role of interleukin 2 (IL 2) in the proliferation of Theileria annulata-infected bovine lymphocytes.
January 1987, Parasitology research,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Bovine helper T-cell clones specific for lymphocytes infected with Theileria parva (Muguga).
July 1987, Parasite immunology,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
January 1993, Veterinary immunology and immunopathology,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Growth characteristics of Theileria parva-infected bovine lymphoblast cultures.
September 1971, American journal of veterinary research,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Serum-free in vitro cultivation of Theileria annulata and Theileria parva schizont-infected lymphocytes.
March 2020, Transboundary and emerging diseases,
T M Coquerelle, and M Eichhorn, and N S Magnuson, and R Reeves, and R O Williams, and D A Dobbelaere
Proliferation of Theileria annulata and Theileria parva macroschizont-infected bovine cells in scid mice.
February 1993, International journal for parasitology,
Copied contents to your clipboard!