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The lactate dehydrogenase (LDH) activity and LDH isozyme ratio in the perfusate were investigated in perfused dog kidneys, preserved from dogs subjected to warm ischemia of varying duration prior to kidney removal. The length of this warm ischemic time exerted a marked effect on the variations in both the LDH activity and isozyme ratio. Moreover, the variations in LDH activity and isozyme ratio during the preservation were found to correlate well. On this basis, the M-component activity of the LDH isozymes and the activity of the LDH4 and LDH5 isoenzymes are proposed to represent more sensitive parameters for evaluating the tissue hypoxic damage. Our data were also analyzed retrospectively, in relation to the ability of the perfused kidney to function on autotransplantation. It was found that the amount of LDH4 and LDH5 isoenzyme activity was of great value in determining the viability of the kidney. Thus, an analysis of the LDH activity in the wash solution can provide considerable and precise information about the kidney viability as a graft.
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
January 1975,
Langenbecks Archiv fur Chirurgie,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
October 1978,
Zeitschrift fur Urologie und Nephrologie,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
January 1983,
Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
August 1983,
Archives of surgery (Chicago, Ill. : 1960),
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
February 1978,
Transplantation,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
March 1973,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
March 1971,
Transplantation,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
November 1974,
Zeitschrift fur Urologie und Nephrologie,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
November 1971,
The New England journal of medicine,
H Amemiya, and
S Suzuki, and
S Niiya, and
H Watanabe, and
S Ohara, and
Y Kaneko, and
K Yoshida, and
Y Motomiya, and
Y Hirao, and
E Okajima
January 1976,
Scandinavian journal of urology and nephrology,
