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The concentrations of the free fatty acids, the triglycerides, the glycerol, and the phospholipids were measured in the perfusate of 10 dog kidneys after hypothermic pulsatile preservation of 48 hours duration. The concentration in free fatty acids decreased in which the fatty acid pattern is unchanged essentially. The free glycerol and the total phospholipids increased. These characteristics of the perfusate refer to an increased lipolysis of neutral fats and a changed membrane composition of the kidney during the preservation.
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