Biomaterial Database

The expression of acetylated microtubules during axonal and dendritic growth in cerebellar macroneurons which develop in vitro. 1989

A Ferreira, and A Cáceres

Laboratorio de Neurobiología, Instituto de Investigación Médica Mercedes y Martín Ferreyra, Córdoba, Argentina.

The distribution of acetylated microtubules in cerebellar macroneurons which develop in culture was studied with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. In these neurons 6-11B-1 helps to define a subset of stable, colchicine-resistant, microtubules that during early neuronal development are exclusively localized in the neuron's axon. On the other hand, in mature neurons acetylated microtubules display a widespread distribution being localized in the axon and thick dendritic trunks, a phenomenon correlated with an increase in the levels of acetylated alpha-tubulin and colchicine-resistant microtubules. Taken collectively, these observations suggest that the acetylation of alpha-tubulin is important for differentiating microtubules during neurite growth: in young neurons this post-translational modification may contribute to determine a selective stabilization of microtubules accompanying axonal differentiation.

UI	MeSH Term	Description	Entries
D008870	Microtubules	Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.	Microtubule
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002531	Cerebellum	The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.	Cerebella,Corpus Cerebelli,Parencephalon,Cerebellums,Parencephalons
D003712	Dendrites	Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS.	Dendrite
D000107	Acetylation	Formation of an acetyl derivative. (Stedman, 25th ed)	Acetylations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon
D014404	Tubulin	A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.	alpha-Tubulin,beta-Tubulin,delta-Tubulin,epsilon-Tubulin,gamma-Tubulin,alpha Tubulin,beta Tubulin,delta Tubulin,epsilon Tubulin,gamma Tubulin