Ceruloplasmin gene expression profile changes in the rat mammary gland during pregnancy, lactation and involution. 2017

Natalia A Platonova, and Iurii A Orlov, and Sergey A Klotchenko, and Victor S Babich, and Ekaterina Y Ilyechova, and Polina S Babich, and Yuri P Garmai, and Andrey V Vasin, and Nadezhda V Tsymbalenko, and Liudmila V Puchkova
Institute of Experimental Medicine, Pavlova str., 12, St., Petersburg 197376, Russia.

Copper metabolism disturbances in mammary gland (MG) cells have severe consequences in newborns. The mechanism that controls the balance of copper in the MG has not been thoroughly characterized. Four primary copper homeostasis genes in mammals: (1) ceruloplasmin (Cp) encoding multifunction multicopper blue (ferr)oxidase; (2) CTR1 encoding high affinity copper importer 1; and (3 and 4) two similar genes encoding Cu(I)/Cu(II)-ATPases P1 type (ATP7A and ATP7B) responsible for copper efflux from the cells and metallation of cuproenzymes formed in the Golgi complex are expressed in MG. This study aimed to characterize expression of these genes during pregnancy, lactation and forced involution in the rat MG. We found that Cp anchored to the plasma membrane and ATP7A were expressed during pregnancy and lactation. Soluble Cp and ATP7B were highly expressed in lactating MG decreasing to its ending. CTR1 activity increased during MG growth and reached its maximum at postpartum and then it decreased until the end of lactation. During early forced MG involution, Cp gene expression persisted; while a form of Cp that lacked exon 18 appeared. We suggest that Cp gene expressional changes at the transcriptional and posttranscriptional level reflect various physiological functions of Cp proteins during MG remodeling.

UI MeSH Term Description Entries
D007774 Lactation The processes of milk secretion by the maternal MAMMARY GLANDS after PARTURITION. The proliferation of the mammary glandular tissue, milk synthesis, and milk expulsion or let down are regulated by the interactions of several hormones including ESTRADIOL; PROGESTERONE; PROLACTIN; and OXYTOCIN. Lactation, Prolonged,Milk Secretion,Lactations, Prolonged,Milk Secretions,Prolonged Lactation,Prolonged Lactations
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002570 Ceruloplasmin A multi-copper blood FERROXIDASE involved in iron and copper homeostasis and inflammation. Caeruloplasmin,Ferroxidase,Ceruloplasmin Ferroxidase,Ceruloplasmin Oxidase,Ferroxidase I,alpha(2)-Ceruloplasmin,Ferroxidase, Ceruloplasmin,Oxidase, Ceruloplasmin
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000073840 Copper-Transporting ATPases P-type ATPases which transport copper ions across membranes in prokaryotic and eukaryotic cells. They possess a conserved CYSTEINE-HISTIDINE-SERINE (CPx) amino acid motif within their transmembrane helices that functions in cation translocation and catalytic activation, and an N-terminal copper-binding CxxC motif that regulates enzyme activity. They play essential roles in intracellular copper homeostasis through regulating the uptake, efflux and storage of copper ions, and in cuproprotein biosynthesis. ATP7B Cu-Binding P Type ATPase,ATPase, Cu++ Transporting, beta Polypeptide (Wilson Disease),Copper-Transporting ATPase,Copper-Transporting ATPase 1,Copper-Transporting ATPase 2,Copper-Transporting Adenosine Triphosphatases,Cu(+)-Transporting ATPases,Cu-Transporting ATPases,Menkes Disease-Associated Protein,Pineal Night-Specific ATPase,Wilson Disease Cu-Binding P Type ATPase,ATP7A Protein,ATPase Copper Transporting alpha,Copper Pump 1,PINA Enzyme,Wilson Disease Protein,ATP7B Cu Binding P Type ATPase,ATPase 1, Copper-Transporting,ATPase 2, Copper-Transporting,ATPase, Copper-Transporting,ATPase, Pineal Night-Specific,ATPases, Copper-Transporting,ATPases, Cu-Transporting,Adenosine Triphosphatases, Copper-Transporting,Copper Transporting ATPase,Copper Transporting ATPase 1,Copper Transporting ATPase 2,Copper Transporting ATPases,Copper Transporting Adenosine Triphosphatases,Cu Transporting ATPases,Menkes Disease Associated Protein,Night-Specific ATPase, Pineal,Pineal Night Specific ATPase,Triphosphatases, Copper-Transporting Adenosine,Wilson Disease Cu Binding P Type ATPase
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D042361 Mammary Glands, Human Glandular tissue in the BREAST of human that is under the influence of hormones such as ESTROGENS; PROGESTINS; and PROLACTIN. In WOMEN, after PARTURITION, the mammary glands secrete milk (MILK, HUMAN) for the nourishment of the young. Human Mammary Gland,Human Mammary Glands,Mammary Alveoli, Human,Mammary Ducts, Human,Mammary Epithelia, Human,Mammary Epithelium, Human,Mammary Gland,Mammary Glands,Mammary Lobules, Human,Alveoli, Human Mammary,Alveolus, Human Mammary,Duct, Human Mammary,Ducts, Human Mammary,Epithelia, Human Mammary,Epithelium, Human Mammary,Gland, Human Mammary,Gland, Mammary,Glands, Human Mammary,Glands, Mammary,Human Mammary Alveoli,Human Mammary Alveolus,Human Mammary Duct,Human Mammary Ducts,Human Mammary Epithelia,Human Mammary Epithelium,Human Mammary Lobule,Human Mammary Lobules,Mammary Alveolus, Human,Mammary Duct, Human,Mammary Gland, Human,Mammary Lobule, Human

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