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DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. 2017
Martin Harterink, and
Marta Esteves da Silva, and
Lena Will, and
Julia Turan, and
Adiljan Ibrahim, and
Alexander E Lang, and
Eljo Y van Battum, and
R Jeroen Pasterkamp, and
Lukas C Kapitein, and
Dmitri Kudryashov, and
Ben A Barres, and
Casper C Hoogenraad, and
J Bradley Zuchero
Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
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