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A direct radioimmunoassay for measuring plasma levels of oestrone sulphate has been developed using 8-anilino-2-naphthalene sulphonic acid to displace oestrone sulphate from plasma binding proteins. Oestrone sulphate was assayed by using an antiserum raised against glucuronide which cross-reacted 100% with oestrone sulphate. The direct assay gave a good analytical recovery of oestrone sulphate and there was a good correlation (r = 0.82, P less than 0.001) for plasma levels of oestrone sulphate measured by the direct assay and a method involving steroid conjugate extraction and enzyme hydrolysis. The mean (+/- S.D.) plasma level of oestrone sulphate in men was 1100 +/- 280 pg/ml. The effect of taking the antibiotic, Ampicillin, on plasma levels of oestrone sulphate was investigated in four men. Plasma levels of oestrone sulphate were significantly reduced after taking Ampicillin for 5 days. Ampicillin may act to lower plasma levels of oestrone sulphate by reducing the growth of bacteria in the gut or by inhibiting oestrogen sulphotransferase activity.
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
January 1982,
The British veterinary journal,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
January 1988,
The British veterinary journal,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
May 1997,
The Journal of endocrinology,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
January 1983,
The British veterinary journal,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
November 1986,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
November 1986,
Acta endocrinologica,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
January 1976,
Current medical research and opinion,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
October 1982,
The Journal of endocrinology,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
November 1972,
The Journal of endocrinology,
M J Reed, and
M M Gompels, and
P A Beranek, and
V H James
September 1977,
The Biochemical journal,
