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Epigenetic changes of histone deacetylation in murine oocytes matured in vitro versus in vivo. 2017

J Huang, and C-H Ding, and Z-Y Li, and X-B Zhang, and Z-S You, and C-Q Zhou, and Y-W Xu
Center of Reproductive Medicine, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People's Republic of China. zhoucanquan@hotmail.com.

In this study, we studied changes in histone acetylation during mouse oocytes meiosis. The aim was to investigate HDAC1 expression patterns in the mouse oocytes and effects of in-vitro maturation on epigenetic modifications during meiosis. Immature and mature oocytes were collected from female Kunming white mice of 4-6 weeks in age. Dynamic changes of histone H3K9, H4K12 acetylation were explored. HDAC1 spatial and temporal expression patterns during meiosis and their expression changes in in-vitro maturation were determined. It was found that histone H3K9 and H4K12 acetylations were gradually disappeared during the meiotic maturation of mouse oocytes. HDAC1 proteins were localized mainly throughout the nucleoplasm in GV-intact oocyte, and colocalized with chromosomes at metaphase II (MII). The acetylated H3K9 and H4K12 were absent in oocytes matured in vivo, while the elevated acetylation of H3K9 and H4K12 was detected in oocytes matured in vitro. When cultured in vitro, the decrease of HDAC1 protein level and mRNA level were observed compared with oocytes matured in vivo. the acetylation of H3K9, H4K12 decreased gradually to undetectable during oocyte meiosis. The histone deacetylation in oocytes was inadequate during in vitro maturation, and the in vitro maturation might lead to reduced HDAC1 expression in oocytes.

UI MeSH Term Description Entries
D008540 Meiosis A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells. M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D009866 Oogenesis The process of germ cell development in the female from the primordial germ cells through OOGONIA to the mature haploid ova (OVUM). Oogeneses
D005260 Female Females
D006657 Histones Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. Histone,Histone H1,Histone H1(s),Histone H2a,Histone H2b,Histone H3,Histone H3.3,Histone H4,Histone H5,Histone H7
D000107 Acetylation Formation of an acetyl derivative. (Stedman, 25th ed) Acetylations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D044127 Epigenesis, Genetic A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression. Epigenetic Processes,Epigenetic Process,Epigenetics Processes,Genetic Epigenesis,Process, Epigenetic,Processes, Epigenetic,Processes, Epigenetics
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

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