-transparent.png){kind=logo}
Cytolysis of target cells by Entamoeba histolytica is a rapid, contact-dependent event that appears to involve calcium and amebic phospholipase A enzymes. There are two phospholipase A enzymes of E. histolytica: one calcium independent and optimally active at an acid pH, and a second calcium dependent and most active at an alkaline pH. The amebic calcium-dependent enzyme was inhibited by pharmacological antagonists that have been shown to reduce cytolysis of target cells by intact amebae: phosphatidylcholine (10(-3) M), Rosenthal's inhibitor (dimethyl-dl-2,3-distearoylpropyl-2'-hydroxyethyl ammonium acetate; 10(-4) M), quinacrine (10(-4) M), and hydrocortisone (10(-4) M). Calcium-independent phospholipase A activity was inhibited by Rosenthal's inhibitor (10(-4) M) and quinacrine (10(-3) M). The calcium-dependent phospholipase A enzyme is highly associated with plasma membrane fractions; the calcium-independent enzyme is predominantly found in soluble fractions. These findings further suggest an association of calcium-dependent phospholipase A activity with cytolytic activity of E. histolytica.
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
December 1967,
Nature,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
January 1969,
Bulletin of the World Health Organization,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
January 2000,
Archives of medical research,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
April 2002,
The Journal of parasitology,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
January 1990,
Archivos de investigacion medica,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
September 1956,
Nature,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
February 1959,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
April 1986,
Molecular and biochemical parasitology,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
January 1995,
Parasitology research,
S A Long-Krug, and
K J Fischer, and
R M Hysmith, and
J I Ravdin
January 1990,
Archivos de investigacion medica,
