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Identification of Entamoeba histolytica intracellular phospholipase A and lysophospholipase L1 activities. 1995
J Vargas-Villarreal, and
H Martínez-Rodríguez, and
J Castro-Garza, and
B D Mata-Cárdenas, and
M T González-Garza, and
S Said-Fernández
División de Biología Celular y Molecular, Instituto Mexicano del Seguro Social, Monterrey, N.L. México.
Entamoeba histolytica phospholipase A and lysophospholipase activities from a vesicular subcellular fraction (P30) were analyzed. The products, obtained using specific substrates labeled with 14C or 3H, indicated the presence of phospholipase A1 and A2 as well as lysophospholipase L1 activities. The enzymes detected could participate in phospholipid metabolism and the alkaline phospholipase A2 may contribute to E. histolytica cytopathogenicity.
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