Comparison of the results of asbestos fibre dust counts in lung tissue obtained by analytical electron microscopy and light microscopy. 1986

F D Pooley, and D L Ranson

The efficiency of the light microscope with that of the electron microscope in detecting asbestos fibres in human lung tissue was computed. Necropsy material from 55 patients who had died from asbestos related diseases was analysed independently by phase contrast microscopy and electron microscopy. As expected the number of fibres identified using electron microscopy was higher than that identified by light microscopy. By adjusting the electron allow for the limited resolving power of the light microscope, however, a significant correlation of the number of fibres identified using the two methods was obtained. The best correlation was found with specimens containing crocidolite (correlation coefficient 0.79) and amosite (correlation coefficient 0.74), while chrysotile gave a much lower correlation (correlation coefficient 0.15). The cumulated fibre diameter distribution obtained using the electron microscope suggests that the light microscope is able to visualise only 5% of crocidolite, 26.5% of amosite, and 0.14% of chrysotile present in lung tissue. Therefore, although it is possible, using the electron microscope, to predict the asbestos fibre count that would be obtained by light microscopy, the reserve prediction cannot be made: it is impossible to determine the proportion of the various asbestos mineral types using the light microscope.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008858 Microscopy, Phase-Contrast A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate. Phase-Contrast Microscopy,Microscopies, Phase-Contrast,Microscopy, Phase Contrast,Phase Contrast Microscopy,Phase-Contrast Microscopies
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001194 Asbestos Asbestos. Fibrous incombustible mineral composed of magnesium and calcium silicates with or without other elements. It is relatively inert chemically and used in thermal insulation and fireproofing. Inhalation of dust causes asbestosis and later lung and gastrointestinal neoplasms.
D017632 Asbestos, Serpentine A type of asbestos that occurs in nature as the dihydrate of magnesium silicate. It exists in two forms: antigorite, a plated variety, and chrysotile, a fibrous variety. The latter makes up 95% of all asbestos products. (From Merck Index, 11th ed, p.893) Chrysotile,Serpentine (Mineral),Serpentine Asbestos,Antigorite,Asbestos, Serpentine, Chrysotile (Mg3(OH)4(Si2O5))
D017638 Asbestos, Crocidolite A lavender, acid-resistant asbestos. Crocidolite Asbestos,Asbestos, Crocidolite (Fe2Mg3Na2(SiO3)8),Asbestos, Crocidolite (Fe5Na2(SiO3)8),Blue Asbestos,Crocidolite,Asbestos, Blue
D017639 Asbestos, Amosite Asbestos, grunerite. A monoclinic amphibole form of asbestos having long fibers and a high iron content. It is used in insulation. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Amosite Asbestos,Amosite,Cummingtonite-Grunerite,Cummingtonite Grunerite

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