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A method is described for handling leucocytes from an inflammatory peritoneal exudate prior to electron microscopy, which allows differential counts from ultra-thin sections to be made. The results of counts from ultrathin sections, viewed on the electron microscope, are compared with samples from the same cell populations prepared on a cytocentrifuge and counted by light microscopy. The results from several cell populations of widely different compositions show clearly that with suitable care taken over preparation and orientation, ultrathin sections can yield comparable differential counts to those obtained by standard light microscope procedures. Possible sources of error are discussed and the advantages of ultrastructural counting assessed. The method has a wide application wherever accurate differential counts are required from cell suspensions processed for electron microscopy.
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
March 1986,
Journal of clinical pathology,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
June 1995,
Clinical and laboratory haematology,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
May 1950,
Journal of clinical pathology,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
July 1907,
The Hospital,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
September 1907,
The Hospital,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
November 1988,
Journal of clinical pathology,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
September 1974,
British journal of haematology,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
September 1971,
Lancet (London, England),
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
April 1981,
Equine veterinary journal,
R Gillett, and
P M Hancock, and
D M Williams, and
M W Hill
January 2014,
Methods in molecular biology (Clifton, N.J.),
