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Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis. 2017
Anne Piantadosi, and
Shibani S Mukerji, and
Pooja Chitneni, and
Tracey A Cho, and
Lisa A Cosimi, and
Deborah T Hung, and
Marcia B Goldberg, and
Pardis C Sabeti, and
Daniel R Kuritzkes, and
Yonatan H Grad
Division of Infectious Disease, Department of Medicine.
Enteroviruses cause a wide spectrum of clinical disease. In this study, we describe the case of a young man with orchitis and aseptic meningitis who was diagnosed with enterovirus infection. Using unbiased "metagenomic" massively parallel sequencing, we assembled a near-complete viral genome, the first use of this method for full-genome viral sequencing from cerebrospinal fluid. We found that the genome belonged to the subgroup echovirus 30, which is a common cause of aseptic meningitis but has not been previously reported to cause orchitis.
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