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A kinetic assay for measuring gamma-glutamyl transpeptidase (GGT) activity has been adapted to microtiter plates and an automated microtiter plate reader. This method permits the simultaneous analysis of enzyme activity in a large number of samples incubated with the chromogenic GGT substrate gamma-glutamyl-p-nitroanilide. A major advantage of this assay over previously reported methods is the substantial reduction in the time needed for measuring sample enzyme activity. In addition, reduction of the total assay volume to 0.28 ml conserves both sample and reagents. This method has been calibrated at 23 degrees C using purified GGT, and used to analyze GGT activity in human sera. The assay is sensitive over a range of 3-200 U/liter.
P M Silber, and
A J Gandolfi, and
K Brendel
May 1985,
Analytical biochemistry,
P M Silber, and
A J Gandolfi, and
K Brendel
January 1990,
Annals of the New York Academy of Sciences,
P M Silber, and
A J Gandolfi, and
K Brendel
December 1970,
Lancet (London, England),
P M Silber, and
A J Gandolfi, and
K Brendel
April 1972,
Deutsche medizinische Wochenschrift (1946),
P M Silber, and
A J Gandolfi, and
K Brendel
January 1981,
Methods in enzymology,
P M Silber, and
A J Gandolfi, and
K Brendel
January 1975,
Advances in clinical chemistry,
P M Silber, and
A J Gandolfi, and
K Brendel
August 1973,
Deutsche medizinische Wochenschrift (1946),
P M Silber, and
A J Gandolfi, and
K Brendel
June 1967,
Polski tygodnik lekarski (Warsaw, Poland : 1960),
P M Silber, and
A J Gandolfi, and
K Brendel
January 1967,
Postepy higieny i medycyny doswiadczalnej,
P M Silber, and
A J Gandolfi, and
K Brendel
February 1995,
Nihon rinsho. Japanese journal of clinical medicine,
