Biomaterial Database

Adaptation of the dye-binding protein assay to microtiter plates. 1985

M G Redinbaugh, and W H Campbell

The dye-binding protein assay has been adapted for use with microtiter plates and a plate reader. The total volume of the assay was reduced to 0.2 ml, with equal volumes of sample and diluted dye reagent being used. Because of the small volume, the assay is conservative of both protein sample and reagent. The use of microtiter plates allows the absorbance of samples to be read using a plate reader, markedly reducing the time needed for evaluating sample absorbances. This assay is quite sensitive, with a limit of less than 0.5 micrograms bovine serum albumin. In addition, the assay has been calibrated with homogeneous nitrate reductase. The response of nitrate reductase in the assay is about 70% of that of the standard protein, bovine serum albumin. The response of several other proteins was evaluated and the differences in response are discussed.

UI	MeSH Term	Description	Entries
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D009565	Nitrate Reductases	Oxidoreductases that are specific for the reduction of NITRATES.	Reductases, Nitrate
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D004396	Coloring Agents	Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.	Coloring Agent,Dye,Dyes,Organic Pigment,Stain,Stains,Tissue Stain,Tissue Stains,Organic Pigments,Pigments, Inorganic,Agent, Coloring,Inorganic Pigments,Pigment, Organic,Pigments, Organic,Stain, Tissue,Stains, Tissue
D012710	Serum Albumin, Bovine	Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)	Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D050901	Nitrate Reductase	An enzyme that catalyzes the oxidation of nitrite to nitrate. It is a cytochrome protein that contains IRON and MOLYBDENUM.	Quinol-Nitrate Oxidoreductase,Respiratory Nitrate Reductase,Nitrate Reductase, Respiratory,Oxidoreductase, Quinol-Nitrate,Quinol Nitrate Oxidoreductase,Reductase, Nitrate,Reductase, Respiratory Nitrate