Biomaterial Database

Polymer-Based Purification of Extracellular Vesicles. 2017

Peter N Brown, and Hang Yin

Drug Discovery Programme, Beatson Institute for Cancer Research, Bearsden, Glasgow, G61 1BD, UK.

Lipid particles, including exosomes (extracellular vesicles [EVs]), are released from cells in vivo and in vitro. The contents of these EVs can be indicative of disease and therefore be utilized in diagnostic biophysical or biochemical assays. To make use of EVs in this way, methods for purification and quantification are required which vary depending on their particles origin and concentration. This chapter provides an overview of EV purification techniques and provides detailed instructions on the purification of EVs by polymer based precipitation and subsequent quantification. The subsequent quantification and characterization of these EVs also presents a challenge, as limited methods are capable of detecting EVs due to their small size.

UI	MeSH Term	Description	Entries
D011108	Polymers	Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).	Polymer
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000067128	Extracellular Vesicles	Membrane limited structures derived from cell membranes and cytoplasmic material, and released into EXTRACELLULAR SPACE. They circulate through the EXTRACELLULAR FLUID and through the peripheral blood in the MICROVASCULATURE where cells, much larger, cannot, thereby affecting a variety of intercellular communication processes.	Apoptotic Bodies,Exovesicles,Apoptotic Body,Bodies, Apoptotic,Body, Apoptotic,Exovesicle,Extracellular Vesicle,Vesicle, Extracellular,Vesicles, Extracellular
D014461	Ultracentrifugation	Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D045744	Cell Line, Tumor	A cell line derived from cultured tumor cells.	Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D055354	Exosomes	A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.