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Woodchucks free from woodchuck hepatitis virus were treated with diethylnitrosamine in vivo for 2 months, and then hepatocytes obtained by enzymatic perfusion were cultured with the hepatopromoter phenobarbital. This in vivo-in vitro procedure gave rise to proliferating epithelial cell foci, from one of which the presently described hepatocyte cell line (WLC-3) was established and characterized. WLC-3 cells possess morphological and biochemical features of differentiated hepatocytes, including glucose-6-phosphatase activity and albumin production. Histopathological analysis of the tumor which developed transitorily in nude mouse subcutis after inoculation of the cell line revealed glandular structures comprising cells of hepatocellular-like morphology. This is the first established woodchuck hepatocyte cell line free from woodchuck hepatitis virus and is therefore expected to be useful for studying the mode of gene expression and viral proliferation of woodchuck hepatitis virus and the mechanisms underlying woodchuck hepatitis virus-related hepatocarcinogenesis.
H Lee, and
T Kawaguchi, and
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T Kitagawa
March 1988,
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H Lee, and
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January 1985,
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H Lee, and
T Kawaguchi, and
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January 1988,
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H Lee, and
T Kawaguchi, and
K Nomura, and
T Kitagawa
October 2009,
In vitro cellular & developmental biology. Animal,
H Lee, and
T Kawaguchi, and
K Nomura, and
T Kitagawa
December 2012,
Biotechnology letters,
H Lee, and
T Kawaguchi, and
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T Kitagawa
November 1997,
Experimental cell research,
H Lee, and
T Kawaguchi, and
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T Kitagawa
November 1986,
Carcinogenesis,
H Lee, and
T Kawaguchi, and
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T Kitagawa
September 1998,
British journal of haematology,
H Lee, and
T Kawaguchi, and
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T Kitagawa
March 2024,
Cell biology and toxicology,
H Lee, and
T Kawaguchi, and
K Nomura, and
T Kitagawa
January 1998,
Investigative ophthalmology & visual science,
