BIOMAT Database Logo BIOMAT Database Logo

Establishment of a new cell line from a woodchuck hepatocellular carcinoma. 1988

S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

A new cell line derived from a woodchuck hepatitis surface antigen-positive woodchuck hepatocellular carcinoma has been established and named T3-HEP-W1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture condition was completed after 3 months, with the doubling time of 24 hr. The morphologic features of the cell by light microscopy were of an epithelial type. The modal chromosome number was 100. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was negative. Integration of woodchuck hepatitis virus DNA was demonstrated by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. T3-HEP-W1 is quite different from the previously reported WH257GE10 cell line and provides another in vitro model for the study of human hepatocellular carcinoma related to hepatitis B virus.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008114 Liver Neoplasms, Experimental Experimentally induced tumors of the LIVER. Hepatoma, Experimental,Hepatoma, Morris,Hepatoma, Novikoff,Experimental Hepatoma,Experimental Hepatomas,Experimental Liver Neoplasms,Hepatomas, Experimental,Neoplasms, Experimental Liver,Experimental Liver Neoplasm,Liver Neoplasm, Experimental,Morris Hepatoma,Novikoff Hepatoma
D008297 Male Males
D008392 Marmota A genus of Sciuridae consisting of 14 species. They are shortlegged, burrowing rodents which hibernate in winter. Woodchucks,Marmots,Marmot,Marmotas,Woodchuck
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006518 Hepatitis Viruses Any of the viruses that cause inflammation of the liver. They include both DNA and RNA viruses as well viruses from humans and animals. Hepatitis Virus, Marmoset,Hepatitis Virus,Hepatitis Viruses, Marmoset,Marmoset Hepatitis Virus,Marmoset Hepatitis Viruses,Virus, Hepatitis,Virus, Marmoset Hepatitis,Viruses, Hepatitis,Viruses, Marmoset Hepatitis
D006524 Hepatitis, Viral, Animal INFLAMMATION of the LIVER in animals due to viral infection. Viral Hepatitis, Animal,Animal Viral Hepatitides,Animal Viral Hepatitis,Viral Hepatitides, Animal
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral

Related Publications

S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment of a cell line from a woodchuck hepatocellular carcinoma.
January 1985, Hepatology (Baltimore, Md.),
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment and characterization of a woodchuck hepatocellular carcinoma cell line (WH44KA).
March 1988, Japanese journal of cancer research : Gann,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment and characterization of a new human hepatocellular carcinoma cell line.
June 1984, In vitro,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment of a cell line (HCC-M) from a human hepatocellular carcinoma.
August 1983, International journal of cancer,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment and characterization of a new Chinese hepatocellular carcinoma cell line, Hep-X1.
January 2023, Human cell,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment of a cell line from a hepatocellular carcinoma from a patient with hemochromatosis.
July 1994, Hepatology (Baltimore, Md.),
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Establishment of a new cell line from maxillary sinus carcinoma.
January 1980, The Annals of otology, rhinology, and laryngology,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Analysis of the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10).
November 1987, International journal of cancer,
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Hepatocellular carcinoma after thorotrast exposure: establishment of a new cell line (Mz-Hep-1).
January 1985, Hepatology (Baltimore, Md.),
S Ohnishi, and H Aoyama, and J Shiga, and Y Itai, and T Moriyama, and T Ishikawa, and N Sasaki, and K Yamamoto, and K Koshimizu, and S Kaneko
Chromosomal assignment of woodchuck hepatitis virus (WHV) DNA integration sites in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10).
April 1989, International journal of cancer,
Copied contents to your clipboard!