Adding functionality to milk-based protein: Preparation, and physico-chemical characterization of β-lactoglobulin-phenolic conjugates. 2018

Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
Department of Engineering, Aarhus University, Gustav Wieds vej 10, Aarhus 8000, Denmark; Department of Dairy Science, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. Electronic address: ahmed.ali@eng.au.dk.

Multi-functional phenolic emulsifiers were prepared by covalently coupling β-Lactoglobulin (βLg) to caffeic acid (CA) using crosslinker chemistry at different pH conditions (pH 2.5, 6.0, and 8.5). The resulting bioconjugates were characterized by MALDI-TOF MS, differential scanning calorimetry (DSC), fluorescence-quenching, infrared and circular dichroism spectroscopies. Furthermore, the emulsifying and antioxidant properties of βLg-CA conjugates were evaluated and compared to native β-Lactoglobulin and the non-covalent β-lactoglobulin/caffeic complex (βLg/CA). Results showed: 1) An optimal molar ratio (8:1) of caffeic acid to βLg was obtained at pH 6; 2) DPPH activity of βLg-CA increases as the number of CA units coupled increases; 3) βLg-CA conjugates displayed comparable or superior water solubility than native βLg and βLg/CA. Moreover, DSC results showed that coupling of CA with βLg significantly increased the thermal stability of βLg. In summary, βLg-CA conjugates can act as effective antioxidant emulsifiers and stabilizers and may find application in food and cosmetic industries.

UI MeSH Term Description Entries
D007782 Lactoglobulins Globulins of milk obtained from the WHEY. Lactoglobulin,beta-Lactoglobulin,beta-Lactoglobulin A,beta-Lactoglobulin B,beta-Lactoglobulin C,beta-Lactoglobulin E,beta-Lactoglobulin F,beta-Lactoglobulin G,beta-Lactoglobulin I,beta Lactoglobulin,beta Lactoglobulin A,beta Lactoglobulin B,beta Lactoglobulin C,beta Lactoglobulin E,beta Lactoglobulin F,beta Lactoglobulin G,beta Lactoglobulin I
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D008894 Milk Proteins The major protein constituents of milk are CASEINS and whey proteins such as LACTALBUMIN and LACTOGLOBULINS. IMMUNOGLOBULINS occur in high concentrations in COLOSTRUM and in relatively lower concentrations in milk. (Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p554) Milk Protein,Protein, Milk,Proteins, Milk
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D055598 Chemical Phenomena The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes. Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical

Related Publications

Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
February 1969, Archives of biochemistry and biophysics,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
December 1990, Biochemistry international,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
January 2011, Bioscience, biotechnology, and biochemistry,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
August 2012, Journal of dairy science,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
September 2014, Food chemistry,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
June 2014, Journal of agricultural and food chemistry,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
November 2006, Biomacromolecules,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
May 2011, The journal of physical chemistry. B,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
August 1976, European journal of biochemistry,
Ahmed A Abd El-Maksoud, and Ismail H Abd El-Ghany, and Hossam S El-Beltagi, and Sampson Anankanbil, and Chiranjib Banerjee, and Steen V Petersen, and Bianca Pérez, and Zheng Guo
October 1979, Toxicology and applied pharmacology,
Copied contents to your clipboard!