BIOMAT Database Logo BIOMAT Database Logo

Burkholderia cepacia complex in cystic fibrosis in a Brazilian reference center. 2017

Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Department of Pediatrics, School of Medical Sciences, University of Campinas, P.O. Box: 6111, Campinas, SP, 13081-970, Brazil.

The Burkholderia cepacia complex (BCC) can cause a severe decline in lung function in cystic fibrosis (CF). Our objective was to determine the BCC prevalence and to evaluate its clinical impact on CF. Clinical and laboratory variables were determined for CF patients with BCC (Group-A = 50 patients) and without BCC (Group-B = 134 patients). The microorganisms were identified by biochemical tests, the Vitek2®Compact test, recA-PCR and recA-nested-PCR with species-specific primers and DNA sequencing. The patients were evaluated by the Shwachman-Kulczycki score (SKCS), Bhalla score (BS), spirometry and body mass index (BMI). The BCC prevalence was 22.5%. The most common species were Burkholderia multivorans (30%), Burkholderia cepacia (24%), Burkholderia cenocepacia IIIA (10%), B. cenocepacia IIIB (2%) and Burkholderia vietnamiensis (2%). There was difference between the groups in nutritional status (p = 0.02) and general activity (p = 0.026). There was difference in total BS points (p = 0.04) and the following parameters: bronchiectasis severity (p = 0.007), peribronchial thickening (p = 0.013), bronchiectasis extent (p = 0.01) and general aspects of the affected bronchial zone (p = 0.02). The respiratory disorder classifications were as follows: obstructive-4.8% (Group-A) and 23.8% (Group-B); restrictive-9.5% (Group-A and Group-B); obstructive + restrictive-19% (Group-A) and 1.6% (Group-B); and obstructive + restrictive with a decreased forced expiratory flow-47.6% (Group-A) and 30.2% (Group-B) (p = 0.02). Nutritional status was a minor contributing factor to weight, height and BMI in the Group-A (p = 0.02). The BCC prevalence, particularly the prevalence of B. multivorans, was higher in this study. The SKCS, BS, spirometry and nutritional status results showed that BCC has a negative impact on clinical status. Phenotypic methods are useful for the identification of presumptive BCC. The Vitek2®Compact test showed accuracy in BCC identification. PCR, nested-PCR, and recA sequencing showed specificity in BCC species identification.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D008297 Male Males
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D003430 Cross-Sectional Studies Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time. Disease Frequency Surveys,Prevalence Studies,Analysis, Cross-Sectional,Cross Sectional Analysis,Cross-Sectional Survey,Surveys, Disease Frequency,Analyses, Cross Sectional,Analyses, Cross-Sectional,Analysis, Cross Sectional,Cross Sectional Analyses,Cross Sectional Studies,Cross Sectional Survey,Cross-Sectional Analyses,Cross-Sectional Analysis,Cross-Sectional Study,Cross-Sectional Surveys,Disease Frequency Survey,Prevalence Study,Studies, Cross-Sectional,Studies, Prevalence,Study, Cross-Sectional,Study, Prevalence,Survey, Cross-Sectional,Survey, Disease Frequency,Surveys, Cross-Sectional
D003550 Cystic Fibrosis An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION. Mucoviscidosis,Cystic Fibrosis of Pancreas,Fibrocystic Disease of Pancreas,Pancreatic Cystic Fibrosis,Pulmonary Cystic Fibrosis,Cystic Fibrosis, Pancreatic,Cystic Fibrosis, Pulmonary,Fibrosis, Cystic,Pancreas Fibrocystic Disease,Pancreas Fibrocystic Diseases
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia complex in Indian cystic fibrosis patients.
November 2012, The Indian journal of medical research,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia in cystic fibrosis.
March 1995, The New England journal of medicine,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia in cystic fibrosis.
March 1995, The New England journal of medicine,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia in cystic fibrosis.
March 1995, The New England journal of medicine,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia complex: clinical course in cystic fibrosis patients.
December 2015, BMC pulmonary medicine,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia complex infection in patients with cystic fibrosis.
July 2002, Journal of medical microbiology,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Clonal diversity among Burkholderia cepacia complex isolates from cystic fibrosis patients in a reference unit.
December 2013, Enfermedades infecciosas y microbiologia clinica,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia complex: a contraindication to lung transplantation in cystic fibrosis?
September 2001, Transplant infectious disease : an official journal of the Transplantation Society,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Burkholderia cepacia infections in cystic fibrosis.
February 1999, The Pediatric infectious disease journal,
Priscila Dentini, and Fernando Augusto Lima Marson, and Luciana Cardoso Bonadia, and Carmen Sílvia Bertuzzo, and Antônio Fernando Ribeiro, and Carlos Emílio Levy, and José Dirceu Ribeiro
Epidemiology of Burkholderia cepacia complex colonisation in cystic fibrosis patients.
June 2004, The European respiratory journal,
Copied contents to your clipboard!