Biomaterial Database

Regulatory volume decrease by cultured renal cells. 1989

C Knoblauch, and M H Montrose, and H Murer

Institute of Physiology, University of Zurich, Switzerland.

Volume regulatory responses of OK cells (a continuous epithelioid cell line from opossum kidney) are examined by electronic cell sizing and measurements of intracellular pH in cell suspensions. In response to a 40% reduction in osmolality, the cells swell and then subsequently shrink toward their starting volume. This regulatory volume decrease (RVD) is reduced by replacement of Cl- in the medium with acetate. Replacement of Cl- with NO3- accelerates the RVD. The RVD response is inhibited by 1 mM quinine or 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in the medium. The inhibitory effect of 100 microM DIDS (but not 1 mM quinine) is altered by replacement of Cl- by NO3- in the medium. Hypotonic challenge does not induce a DIDS-sensitive net flux of acid-base equivalents. Addition of (9 microM) valinomycin also inhibits the RVD response. It is suggested that the RVD response of OK cells involves activation of separate K+ and Cl- channels.

UI	MeSH Term	Description	Entries
D007038	Hypotonic Solutions	Solutions that have a lesser osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Solutions, Hypotonic
D007424	Intracellular Fluid	The fluid inside CELLS.	Fluid, Intracellular,Fluids, Intracellular,Intracellular Fluids
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009893	Opossums	New World marsupials of the family Didelphidae. Opossums are omnivorous, largely nocturnal and arboreal MAMMALS, grow to about three feet in length, including the scaly prehensile tail, and have an abdominal pouch in which the young are carried at birth.	Didelphidae,Opossum
D011803	Quinine	An alkaloid derived from the bark of the cinchona tree. It is used as an antimalarial drug, and is the active ingredient in extracts of the cinchona that have been used for that purpose since before 1633. Quinine is also a mild antipyretic and analgesic and has been used in common cold preparations for that purpose. It was used commonly and as a bitter and flavoring agent, and is still useful for the treatment of babesiosis. Quinine is also useful in some muscular disorders, especially nocturnal leg cramps and myotonia congenita, because of its direct effects on muscle membrane and sodium channels. The mechanisms of its antimalarial effects are not well understood.	Biquinate,Legatrim,Myoquin,Quinamm,Quinbisan,Quinbisul,Quindan,Quinimax,Quinine Bisulfate,Quinine Hydrochloride,Quinine Lafran,Quinine Sulfate,Quinine Sulphate,Quinine-Odan,Quinoctal,Quinson,Quinsul,Strema,Surquina,Bisulfate, Quinine,Hydrochloride, Quinine,Sulfate, Quinine,Sulphate, Quinine
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D002259	Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone	A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.	Carbonyl Cyanide para-Trifluoromethoxyphenylhydrazone,FCCP,(4-(Trifluoromethoxy)phenyl)hydrazonopropanedinitrile,Carbonyl Cyanide p Trifluoromethoxyphenylhydrazone,Carbonyl Cyanide para Trifluoromethoxyphenylhydrazone,Cyanide p-Trifluoromethoxyphenylhydrazone, Carbonyl,Cyanide para-Trifluoromethoxyphenylhydrazone, Carbonyl,p-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide,para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D006706	Homeostasis	The processes whereby the internal environment of an organism tends to remain balanced and stable.	Autoregulation