Biomaterial Database

Regulatory volume decrease in cultured myoblasts L6. 1995

J Zachar, and O Hurnák

Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.

A method is described for measuring volume changes in single L6 myoblasts at a stage of proliferating "myoballs", which allows to follow volume changes on single cell level by means of quantitative video image analysis. Myoblasts exposed to hypoosmotic or hyperosmotic challenges for up to 3-5 min behave as osmometers. The relative cell volume is a linear function of the reciprocal of the relative osmolality in the range 0.5-2T. Cells exposed to hypotonic Krebs solution with Na+ and Cl- ions as the main ions exhibit volume readjustment towards the original level. The regulatory volume decrease (RVD) was complete after about 15 min in hypotonic solution with Cmax (maximum RVD) increasing with the decrease in osmolality in the test solution. By replacing external Na+ by K+ in the presence of external Cl- regulatory volume decrease was reversed; myoblast volume continued to increase. RVD was present after replacing Cl- with NO3. Quinine (0.5 mmol/l) partially blocked RVD. It is suggested that RVD in L6 myoblasts is mediated mainly by separate K+ and Cl- channels.

UI	MeSH Term	Description	Entries
D006982	Hypertonic Solutions	Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Hypertonic Solution,Solution, Hypertonic,Solutions, Hypertonic
D007038	Hypotonic Solutions	Solutions that have a lesser osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Solutions, Hypotonic
D007477	Ions	An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009994	Osmolar Concentration	The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.	Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015221	Potassium Channels	Cell membrane glycoproteins that are selectively permeable to potassium ions. At least eight major groups of K channels exist and they are made up of dozens of different subunits.	Ion Channels, Potassium,Ion Channel, Potassium,Potassium Channel,Potassium Ion Channels,Channel, Potassium,Channel, Potassium Ion,Channels, Potassium,Channels, Potassium Ion,Potassium Ion Channel
D048429	Cell Size	The quantity of volume or surface area of CELLS.	Cell Volume,Cell Sizes,Cell Volumes,Size, Cell,Sizes, Cell,Volume, Cell,Volumes, Cell