BIOMAT Database Logo BIOMAT Database Logo

Incorporation of a recombinant Eimeria maxima IMP1 antigen into nanoparticles confers protective immunity against E. Maxima challenge infection. 2018

Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Animal Parasitic Diseases Laboratory, Building 1040, NEA, ARS, USDA, Beltsville, MD 20705, United States. Electronic address: mark.jenkins@ars.usda.gov.

The purpose of this study was to determine if conjugating a recombinant Eimeria maxima protein, namely EmaxIMP1, into 20 nm polystyrene nanoparticles (NP) could improve the level of protective immunity against E. maxima challenge infection. Recombinant EmaxIMP1 was expressed in Escherichia coli as a poly-His fusion protein, purified by NiNTA chromatography, and conjugated to 20 nm polystyrene NP (NP-EmaxIMP1). NP-EMaxIMP1 or control non-recombinant (NP-NR) protein were delivered per os to newly-hatched broiler chicks with subsequent booster immunizations at 3 and 21 days of age. In battery cage studies (n = 4), chickens immunized with NP-EMaxIMP1 displayed complete protection as measured by weight gain (WG) against E. maxima challenge compared to chickens immunized with NP-NR. WG in the NP-EMaxIMP1-immunized groups was identical to WG in chickens that were not infected with E. maxima infected chickens. In floor pen studies (n = 2), chickens immunized with NP-EMaxIMP1 displayed partial protection as measured by WG against E. maxima challenge compared to chickens immunized with NP-NR. In order to understand the basis for immune stimulation, newly-hatched chicks were inoculated per os with NP-EMaxIMP1 or NP-NR protein, and the small intestine, bursa, and spleen, were examined for NP localization at 1 h and 6 h post-inoculation. Within 1 h, both NP-EMaxIMP1 and NP-NR were observed in all 3 tissues. An increase was observed in the level of NP-EmaxIMP1 and NP-NR in all tissues at 6 h post-inoculation. These data indicate that 20 nm NP-EmaxIMP1 or NP-NR reached deeper tissues within hours of oral inoculation and elicited complete to partial immunity against E. maxima challenge infection.

UI MeSH Term Description Entries
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D011137 Polystyrenes Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads. Polystyrol,Polystyrene,Polystyrols
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003048 Coccidiosis Protozoan infection found in animals and man. It is caused by several different genera of COCCIDIA. Besnoitiasis,Besnoitiosis,Besnoitiases,Besnoitioses,Coccidioses
D004539 Eimeria A genus of protozoan parasites of the subclass COCCIDIA. Various species are parasitic in the epithelial cells of the liver and intestines of man and other animals. Eimerias
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000953 Antigens, Protozoan Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered. Protozoan Antigens

Related Publications

Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis.
July 2020, Parasites & vectors,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima.
November 2018, BMC veterinary research,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Vaccination with Eimeria tenella elongation factor-1α recombinant protein induces protective immunity against E. tenella and E. maxima infections.
August 2017, Veterinary parasitology,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen.
April 2018, Veterinary parasitology,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits.
October 2022, Parasites & vectors,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Embryo vaccination of chickens using a novel adjuvant formulation stimulates protective immunity against Eimeria maxima infection.
November 2010, Vaccine,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Partial protective of chickens against Eimeria tenella challenge with recombinant EtMIC-1 antigen.
June 2013, Parasitology research,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection.
July 2019, Parasites & vectors,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Protective immunity of recombinant Mycobacterium bovis BCG expressing rhomboid gene against Eimeria tenella challenge.
March 2009, Veterinary parasitology,
Mark C Jenkins, and Laura Stevens, and Celia O'Brien, and Carolyn Parker, and Katrzyna Miska, and Vjollca Konjufca
Mucosal delivery of antigen-coated nanoparticles to lungs confers protective immunity against tuberculosis infection in mice.
February 2014, European journal of immunology,
Copied contents to your clipboard!