-transparent.png){kind=logo}
Hexosaminidases A and B were purified by affinity chromatography from normal gastric mucosa, after preliminary separation of isozymes by anion exchange chromatography. Heparin and mannosamine were coupled to Sepharose 4B and used as affinity matrices and the purified enzymes were found to be homogeneous when analysed by polyacrylamide slab gel electrophoresis. This combination of 2 novel affinity chromatographic procedures is superior to existing methods in that a final yield of over 70% could be achieved. Also, the number of steps required to obtain homogeneity are less in contrast to the conventional methods used previously.
Related Publications
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
September 1974,
FEBS letters,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
April 1977,
The Biochemical journal,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
November 1975,
Israel journal of medical sciences,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
August 1978,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
December 1976,
The Biochemical journal,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
July 1977,
The Biochemical journal,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
December 1978,
Biochemical medicine,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
May 1972,
Canadian journal of biochemistry,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
July 1977,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
P Sankaranarayanan, and
S Chandrasekaran, and
R Puvanakrishnan, and
S C Dhar, and
S Gopalakrishnan, and
N Rangabashyam
January 2012,
Methods in molecular biology (Clifton, N.J.),
