-transparent.png){kind=logo}
High affinity, stereoselective binding sites for [3H]leukotriene E4 ([3H]LTE4) have been identified and characterized in guinea pig lung membranes. [3H]LTE4 bound to these membranes with a pharmacological specificity identical to that previously observed for the [3H]LTD4 receptor in guinea pig lung. [3H]LTE4 specific binding was selectively inhibited by Na+, enhanced by Ca2+, Mg2+ and Mn2+ and modulated by guanine nucleotides. Scatchard analysis of saturation binding data showed a single class of high affinity and saturable binding sites, with a dissociation constant (Kd) of 0.4 +/- 0.2 nM and a density (Bmax) of 430 +/- 50 fmol/mg membrane protein, similar to values observed for the LTD4 receptor in guinea pig lung. The rank order potency of agonist binding to the [3H]LTE4 binding sites was LTD4 greater than LTE4 much greater than LTC4. These results indicate that [3H]LTE4 binds to [3H]LTD4 receptors and suggests that induction of smooth muscle contraction by LTD4 and LTE4 may be mediated by identical mechanisms and receptors in the guinea pig lung.
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
February 1989,
The Journal of pharmacology and experimental therapeutics,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
December 1989,
The Journal of pharmacology and experimental therapeutics,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
February 1987,
Biochemical and biophysical research communications,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
November 1990,
The Journal of pharmacology and experimental therapeutics,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
January 1990,
Molecular pharmacology,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
September 1989,
The American review of respiratory disease,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
August 1984,
Proceedings of the National Academy of Sciences of the United States of America,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
December 1998,
European journal of pharmacology,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
March 1994,
Annals of the New York Academy of Sciences,
S Mong, and
M O Scott, and
M A Lewis, and
H L Wu, and
G K Hogaboom, and
M A Clark, and
S T Crooke
March 1986,
Molecular pharmacology,
