BIOMAT Database Logo BIOMAT Database Logo

First evaluation of endotoxins in veterinary autogenous vaccines produced in Italy by LAL assay. 2018

Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati" Pharmaceutical Unit, Via G. Salvemini 1, 06126, Perugia (PG), Italy.

Endotoxin contamination is a serious concern for manufacturers of biological products and vaccines in terms of not only quality but also safety parameters. We evaluated the endotoxin presence in different veterinary autogenous vaccines produced by the Pharmaceutical Unit at the Experimental Zooprophylactic Institute of Umbria and Marche "Togo Rosati" (IZSUM). According to the 3Rs principles (Replace, Reduce, Refine), which aim to progressively reduce animal use in the quality control process, we tested the vaccines obtained from gram-negative bacteria and adjuvants by the limulus amebocyte lysate (LAL) assay. The results revealed low endotoxin concentrations compared to available data in the literature and represent the first report of the application of the 3Rs principles to veterinary autogenous vaccines production in Italy.

UI MeSH Term Description Entries
D007558 Italy A country in southern Europe, a peninsula extending into the central Mediterranean Sea, northeast of Tunisia. The capital is Rome. Sardinia
D008033 Limulus Test Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals. Limulus Tests,Test, Limulus,Tests, Limulus
D004731 Endotoxins Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells. Endotoxin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000820 Animal Diseases Diseases that occur in VERTEBRATE animals. Diseases, Animal
D054991 Autovaccines Bacterial vaccines prepared from non-pathogenic, autologous bacteria of human origin. In Eastern Europe they are used in humans to treat chronic inflammatory disorders that are resistant to standard treatments. Worldwide they have veterinary use in all types of infectious disease. Autogenous Vaccine,Autogenous Vaccines,Autovaccine,Vaccine, Autogenous,Vaccines, Autogenous

Related Publications

Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Evaluation of veterinary autogenous vaccines safety by an MTT in-vitro cytotoxicity assay.
December 2019, Veterinaria italiana,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Veterinary autogenous vaccines.
January 2004, Developments in biologicals,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Destruction of typical endotoxins by dry heat as determined using LAL assay and pyrogen assay.
January 1993, Journal of parenteral science and technology : a publication of the Parenteral Drug Association,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Studies on Escherichia coli vaccines: estimation of endotoxins in veterinary vaccines.
December 1981, Veterinary research communications,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Design criteria and evaluation of the LAL-4000 for kinetic turbidimetric LAL assay.
January 1987, Progress in clinical and biological research,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
[Use of the Limulus test (LAL) to detect endotoxins].
January 1996, Postepy higieny i medycyny doswiadczalnej,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
[Limulus amoebocyte lysate (LAL) test for demonstration of endotoxins].
May 1980, Ugeskrift for laeger,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
Endotoxins in commercial vaccines.
September 1978, Applied and environmental microbiology,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
The physiological disturbances produced by endotoxins.
January 1954, Annual review of physiology,
Antonella Di Paolo, and Katia Forti, and Lucia Anzalone, and Sara Corneli, and Martina Pellegrini, and Giulio Severi, and Monica Cagiola
The relative activity of endotoxins of different origins in the LAL gelation method and in the LAL chromogenic substrate method.
January 1987, Progress in clinical and biological research,
Copied contents to your clipboard!