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A complete cDNA clone of murine interferon-gamma (MuIFN-gamma) was obtained by recombining two appropriate segments from partial cDNA clones originally identified by colony hybridization with rat IFN-gamma chromosomal gene fragments as probes. An expression vector was constructed in which the cDNA was placed under control of the SV40 early promoter. Transient expression of MuIFN-gamma was obtained by transformation of COS-1 cells. Subsequently, this interferon expression unit was linked to a vector containing a dihydrofolate reductase (DHFR) modular gene and used to transform DHFR(-)-CHO cells. Cell clones were selected that constitutively produce an interferon activity which by several criteria was found to be indistinguishable from natural, splenocyte-derived MuIFN-gamma.
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
December 1989,
Proceedings of the National Academy of Sciences of the United States of America,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
November 1997,
International archives of allergy and immunology,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
November 1989,
Proceedings of the National Academy of Sciences of the United States of America,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
April 1982,
Nucleic acids research,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
October 1983,
Proceedings of the National Academy of Sciences of the United States of America,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
January 1990,
Lymphokine research,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
May 2001,
Current protocols in neuroscience,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
December 1989,
Proceedings of the National Academy of Sciences of the United States of America,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
December 1986,
Journal of leukocyte biology,
R Dijkmans, and
G Volckaert, and
J Van Damme, and
M De Ley, and
A Billiau, and
P De Somer
October 1989,
The Journal of biological chemistry,
