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Starting with mRNA derived from Staphylococcal enterotoxin A induced human splenocytes, dsDNA was synthesized and inserted into unique BamHI site of the eukaryotic expression vector pSV529 (1). A recombinant plasmid containing human immune interferon (IFN-gamma) cDNA was identified by hybridization of plasmid inserted DNA bound onto nitrocellulose filters with mRNA derived from SEA-induced splenocytes, translation of the eluted RNA in Xenopus laevis oocytes and assaying for IFN activity. Plasmids containing the entire human IFN-gamma cDNA sequence were identified by colony hybridization and were sequenced. A unique coding region was identified which predicted a protein of 166 amino acids, the 20 N-terminal amino acids of which presumably represent a signal peptide. After transfection of monkey cells with plasmid DNA isolated from one of the recombinant clones (pHIIF-SV-gamma 1), IFN was excreted into the culture medium. This IFN was not distinguishable from human IFN-gamma by serological criteria or by cell target species specificity.
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
June 1985,
Nucleic acids research,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
July 1980,
Proceedings of the National Academy of Sciences of the United States of America,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
October 1983,
Proceedings of the National Academy of Sciences of the United States of America,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
January 1985,
Journal of interferon research,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
October 1990,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
February 1992,
Bioscience, biotechnology, and biochemistry,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
November 1997,
International archives of allergy and immunology,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
February 1982,
Nature,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
April 1990,
Biochemical and biophysical research communications,
R Devos, and
H Cheroutre, and
Y Taya, and
W Degrave, and
H Van Heuverswyn, and
W Fiers
April 1995,
Molecular immunology,
