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A synthetic gene encoding human insulin-like growth factor I (hIGF-I) was assembled and inserted into an expression vector containing the cytomegalovirus immediate early (CMV-IE) transcriptional regulatory region and portions of the bovine growth hormone gene. The recombinant plasmid encodes a 97 amino acid fusion protein containing the first 27 amino acids of the bovine growth hormone precursor and the 70 amino acids of hIGF-I. This plasmid, when transiently introduced into cultured mouse fibroblasts, directs synthesis of the fusion protein, subsequent proteolytic removal of the bovine growth hormone signal peptide, and secretion of hIGF-I into the culture medium. Conditioned medium from transfected cells inhibits binding of 125I-labeled IGF-I to type I IGF receptors on human placental membranes and to acid-stable human serum carrier proteins. The recombinant hIGF-I produced is biologically active, as monitored by the stimulation of DNA synthesis in vascular smooth muscle cells.
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
July 1989,
The Journal of clinical endocrinology and metabolism,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
June 1995,
Journal of cellular physiology,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
December 1998,
Molecular and cellular biochemistry,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
January 1991,
Cytogenetics and cell genetics,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
March 1991,
Molecular and cellular endocrinology,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
June 1994,
Journal of molecular endocrinology,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
January 1985,
Human genetics,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
January 1983,
Nature,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
May 1993,
Fertility and sterility,
M L Bayne, and
M A Cascieri, and
B Kelder, and
J Applebaum, and
G Chicchi, and
J A Shapiro, and
F Pasleau, and
J J Kopchick
May 1991,
Hypertension (Dallas, Tex. : 1979),
