BIOMAT Database Logo BIOMAT Database Logo

Soluble factors derived from neuroblastoma cell lines suppress dendritic cell differentiation and activation. 2019

Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Department of Medical Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.

Dendritic cells (DC) play a key role in the initiation of both antitumor immunity and immunological tolerance. It has been demonstrated that exposure to soluble factors produced by tumor cells modulates DC functions and induces tolerogenic DC differentiation. In this study, we investigated the effects of neuroblastoma cell line-derived soluble factors on DC differentiation. Monocytes isolated from healthy volunteers were incubated with interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor in the presence of culture supernatants from neuroblastoma cell lines. The culture supernatants from neuroblastoma cell lines, such as NLF and GOTO, partially blocked both downregulation of CD14 and upregulation of CD1a, and dramatically decreased IL-12 and tumor necrosis factor (TNF)-α production from mature DC, while no effect of SH-SY5Y cell supernatant was noted. In addition, IL-6 and IL-10 production from monocytes was increased by the supernatants of NLF and GOTO cells at 24 hours after incubation. Furthermore, we evaluated DC functions through stimulation of invariant natural killer T (iNKT) cells. α-Galactosylceramide-pulsed DC co-cultured with supernatants of NLF cells were unable to sufficiently stimulate iNKT cells. The decreased ability of iNKT cells to produce interferon (IFN)-γ after stimulation with neuroblastoma cell line supernatant-cultured DC was reversed by addition of IL-12. CD40 expression and IL-12 production in NLF-sup-treated DC were increased by addition of exogenous IFN-γ. These results indicate that tolerogenic DC are induced in the neuroblastoma tumor microenvironment and attenuate the antitumor effects of iNKT cells. Interactions between iNKT cells and αGalCer-pulsed DC have the potential to restore the immunosuppression of tolerogenic DC through IFN-γ production.

UI MeSH Term Description Entries
D007371 Interferon-gamma The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D009447 Neuroblastoma A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51) Neuroblastomas
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003713 Dendritic Cells Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION). Dendritic Cells, Interdigitating,Interdigitating Cells,Plasmacytoid Dendritic Cells,Veiled Cells,Dendritic Cells, Interstitial,Dendritic Cells, Plasmacytoid,Interdigitating Dendritic Cells,Interstitial Dendritic Cells,Cell, Dendritic,Cell, Interdigitating,Cell, Interdigitating Dendritic,Cell, Interstitial Dendritic,Cell, Plasmacytoid Dendritic,Cell, Veiled,Cells, Dendritic,Cells, Interdigitating,Cells, Interdigitating Dendritic,Cells, Interstitial Dendritic,Cells, Plasmacytoid Dendritic,Cells, Veiled,Dendritic Cell,Dendritic Cell, Interdigitating,Dendritic Cell, Interstitial,Dendritic Cell, Plasmacytoid,Interdigitating Cell,Interdigitating Dendritic Cell,Interstitial Dendritic Cell,Plasmacytoid Dendritic Cell,Veiled Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015847 Interleukin-4 A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells. B-Cell Growth Factor-I,B-Cell Stimulatory Factor-1,Binetrakin,IL-4,Mast Cell Growth Factor-2,B Cell Stimulatory Factor-1,B-Cell Growth Factor-1,B-Cell Proliferating Factor,B-Cell Stimulating Factor-1,B-Cell Stimulatory Factor 1,BCGF-1,BSF-1,IL4,MCGF-2,B Cell Growth Factor 1,B Cell Growth Factor I,B Cell Proliferating Factor,B Cell Stimulating Factor 1,B Cell Stimulatory Factor 1,Interleukin 4,Mast Cell Growth Factor 2
D016178 Granulocyte-Macrophage Colony-Stimulating Factor An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. CSF-GM,Colony-Stimulating Factor, Granulocyte-Macrophage,GM-CSF,Histamine-Producing Cell-Stimulating Factor,CSF-2,TC-GM-CSF,Tumor-Cell Human GM Colony-Stimulating Factor,Cell-Stimulating Factor, Histamine-Producing,Colony Stimulating Factor, Granulocyte Macrophage,Granulocyte Macrophage Colony Stimulating Factor,Histamine Producing Cell Stimulating Factor,Tumor Cell Human GM Colony Stimulating Factor

Related Publications

Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Differentiation factors from cell lines.
January 1984, Symposium on Fundamental Cancer Research,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Neuroblastoma inhibits dendritic cell differentiation and function.
November 2000, Current surgery,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Dendritic cell derived cytokines in human natural killer cell differentiation and activation.
January 2013, Frontiers in immunology,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
[Morphological and biochemical studies of cell lines derived from human neuroblastoma].
February 1976, Kaibogaku zasshi. Journal of anatomy,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Neuroblastoma-derived gangliosides inhibit dendritic cell generation and function.
January 2001, Cancer research,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
SV40-adenovirus immortalized cell lines derived from mouse lymphoid dendritic cell preparations.
June 1995, Immunology and cell biology,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Elevated interleukin-6 and G-CSF in human pancreatic cancer cell conditioned medium suppress dendritic cell differentiation and activation.
June 2007, Cancer research,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Regulation of human B-cell activation, proliferation, and differentiation by soluble factors.
September 1984, Journal of clinical immunology,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Induction of dendritic cell maturation in the skin microenvironment by soluble factors derived from colon carcinoma.
January 2014, Human vaccines & immunotherapeutics,
Kazuaki Harada, and Fumie Ihara, and Mariko Takami, and Toshiko Kamata, and Naoko Mise, and Hiroko Yoshizawa, and Tomoro Hishiki, and Takeshi Saito, and Keita Terui, and Mitsuyuki Nakata, and Shugo Komatsu, and Takayuki Ikeuchi, and Toshinori Nakayama, and Hideo Yoshida, and Shinichiro Motohashi
Soluble helminth products suppress clinical signs in murine experimental autoimmune encephalomyelitis and differentially modulate human dendritic cell activation.
June 2012, Molecular immunology,
Copied contents to your clipboard!