Biomaterial Database

Effect of high ambient temperature on the lipids and on the activity of enzymes of rat liver microsomes. 1986

A Suliński, and M Jedruch

The work has been aimed at determining the content of cholesterol and selected phospholipids (phosphatidyl choline, phosphatidyl ethanolamine, phosphatidylserine, and phosphatidylinositol) in rats' liver microsomes subjected to single and repeated exposure to an increased ambient temperature. Simultaneously, under the same experimental conditions, NADH activities--cyt. c. reductase and glucoso-6-phosphatase-in liver microsomal fractions were determined. In the experiments--a thermal chamber with controlled temperature, continual airflow and controllable humidity coming to 47-65% was used. Rats were subject to 6-hr exposure to the temperature of 21 degrees C, 28 degrees C, and 35 degrees C singly or for 3 and 6 consecutive days. In the experimental arrangements applied, no significant changes in the content of test phospholipids and cholesterol were found. The slight increase in the PC/PE relation under the effect of 28 degrees C and 35 degrees C for 6 consecutive days was not statistically significant. Cholesterol content in rats subjected to 35 degrees C for 6 days, 6 hrs daily, was higher--compared to the control group exposed to 21 degrees C--by about 15%, this differences being not statistically significant, either. The activities of NADH: cyt. c. reductase and glucoso-6-phosphatase in result of rats' single, 3- or 6-fold exposure to an increased temperature exhibit a level approximating the activity of those enzymes in controls. The lowest activities were observed in rats subjected to 35 degrees C for 6 consecutive days, 6 hrs daily.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008862	Microsomes, Liver	Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.	Liver Microsomes,Liver Microsome,Microsome, Liver
D009251	NADPH-Ferrihemoprotein Reductase	A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.	Cytochrome P-450 Reductase,Ferrihemoprotein P-450 Reductase,NADPH Cytochrome P-450 Oxidoreductase,NADPH Cytochrome P-450 Reductase,NADPH Cytochrome c Reductase,Cytochrome P-450 Oxidase,Cytochrome P450 Reductase,Ferrihemoprotein P450 Reductase,NADPH Cytochrome P450 Oxidoreductase,NADPH Cytochrome P450 Reductase,NADPH-Cytochrome P450 Reductase,NADPH-P450 Reductase,Cytochrome P 450 Oxidase,Cytochrome P 450 Reductase,Ferrihemoprotein P 450 Reductase,NADPH Cytochrome P 450 Oxidoreductase,NADPH Cytochrome P 450 Reductase,NADPH Ferrihemoprotein Reductase,NADPH P450 Reductase,Oxidase, Cytochrome P-450,P-450 Oxidase, Cytochrome,P450 Reductase, Cytochrome,P450 Reductase, NADPH-Cytochrome,Reductase, Cytochrome P-450,Reductase, Cytochrome P450,Reductase, Ferrihemoprotein P-450,Reductase, Ferrihemoprotein P450,Reductase, NADPH-Cytochrome P450,Reductase, NADPH-Ferrihemoprotein,Reductase, NADPH-P450
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D001831	Body Temperature	The measure of the level of heat of a human or animal.	Organ Temperature,Body Temperatures,Organ Temperatures,Temperature, Body,Temperature, Organ,Temperatures, Body,Temperatures, Organ
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D005954	Glucosephosphate Dehydrogenase		Glucose-6-Phosphate Dehydrogenase,Dehydrogenase, Glucose-6-Phosphate,Dehydrogenase, Glucosephosphate,Glucose 6 Phosphate Dehydrogenase
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D050356	Lipid Metabolism	Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.	Metabolism, Lipid