Biomaterial Database

Chromosome condensation and radiation-induced G2 arrest studied by the induction of premature chromosome condensation following cell fusion. 1978

J B Mitchell, and J S Bedford

When mitotic and interphase cells are fused together, the chromosomes of the interphase cell sometimes condense prematurely. The phenomenon of premature chromosome condensation (PCC) was utilized in investigating the problem of whether the chromosomes of cells suffering a radiation-induced G2 delay are capable of condensation. Colcemide-arrested mitotic cells were fused with synchronized G2 cells, and with irradiated cells suffering a G2 delay. The frequency of PCC in mitotic X G2 binucleate cells was determined. This was compared to the PCC frequency in an unirradiated synchronized population rich in G2 cells after fusion with mitotic cells. Flash-labelling with 3HTdR and autoradiography allowed us to eliminate S-phase cells. The frequency of G2 PCCs was not significantly different for the irradiated G2-delayed or unirradiated cells. From these results we conclude that the chromosomes of cells suffering a G2 arrest are capable of condensation, although the involvement of the condensation process in radiation-induced G2 delay cannot be ruled out.

UI	MeSH Term	Description	Entries
D007621	Karyotyping	Mapping of the KARYOTYPE of a cell.	Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D008940	Mitotic Index	An expression of the number of mitoses found in a stated number of cells.	Index, Mitotic,Indices, Mitotic,Mitotic Indices
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002459	Cell Fusion	Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.	Cell Fusions,Fusion, Cell,Fusions, Cell
D002875	Chromosomes	In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Chromosome
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D013936	Thymidine	A nucleoside in which THYMINE is linked to DEOXYRIBOSE.	2'-Deoxythymidine,Deoxythymidine,2' Deoxythymidine