-transparent.png){kind=logo}
In the paper radioimmunosorbent test (PRIST) anti-human IgE coupled paper discs are used for the estimation of total IgE in blood serum: in the radioallergosorbent test (RAST) allergen-coupled paper discs are used for the estimation of specific IgE in blood serum. The bound IgE or specific IgE is quantified by a 125I-labelled anti-human IgE. The non-bound 125I-labelled anti-human IgE can be collected and used in new assay. By a 4-h incubation of the used paper discs with 1 M glycine-HCl buffer (pH 2.7) the IgE-labelled anti-IgE complex can largely be removed. The paper discs treated in this manner can be used in a new assay.
Related Publications
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
September 1981,
Clinical allergy,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
September 1977,
Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
June 1979,
Nihon rinsho. Japanese journal of clinical medicine,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
January 1987,
International archives of allergy and applied immunology,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
February 1979,
Zeitschrift fur Hautkrankheiten,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
November 1985,
Otolaryngologic clinics of North America,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
January 1990,
Dermatologica,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
May 1977,
Monatsschrift fur Kinderheilkunde,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
January 1978,
Journal of immunological methods,
P L Bruynzeel, and
W van den Bogaard, and
L Berrens
September 1976,
Clinical allergy,
