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In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11) and Tp44) and murine (Thy-1, TAP, and Ly-6) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti- variants of the human T-cell line Jurkat with the murine Thy-1.2 gene. Our data indicate that in CD3/Ti-, Thy-1.2+ transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti alpha- or beta-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
January 1988,
Annals of the New York Academy of Sciences,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
June 1991,
Immunology letters,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
May 1989,
European journal of immunology,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
September 1998,
Biochemical and biophysical research communications,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
April 1988,
Journal of immunology (Baltimore, Md. : 1950),
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
June 1981,
European journal of immunology,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
July 1991,
European journal of immunology,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
October 1995,
International immunology,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
June 1986,
Cellular immunology,
K C Gunter, and
R N Germain, and
R A Kroczek, and
T Saito, and
W M Yokoyama, and
C Chan, and
A Weiss, and
E M Shevach
September 1988,
Scandinavian journal of immunology,
