Biomaterial Database

Synthesis, characterization and cytotoxicity of alanine-capped CuS nanoparticles using human cervical carcinoma HeLa cells. 2019

T P Mofokeng, and M J Moloto, and P M Shumbula, and P Tetyana

Department of Chemistry, Vaal University of Technology, Private Bag X021 Vanderbijlpark, 1900, South Africa.

Nanoparticles in biomedicine, requires a detailed understanding of the biocompatibility and toxicity of nanoparticles in human beings. The biological and physicochemical properties of nanoparticles bring new challenges with regards to management of potential adverse health effects following exposure. This work reports on the synthetic optimization of condition of copper sulphide nanoparticles and the cytotoxicity effects of the particles on the human cervical carcinoma cells. CuS nanoparticles synthesized produced a single and different XRD phases in basic and acidic media, respectively. Temperature variation influenced both the optical and morphological properties of the nanoparticles. The highest temperature (95 °C) resulted in particles with superior properties compared to the ones synthesized at lower temperatures. The cytotoxicity tests showed that the particles were nontoxic at low to moderate concentrations and only induced toxicity at higher concentrations. Particles synthesized at 95 °C were less toxic compared to other nanoparticles as determined by their CC50 values.

UI	MeSH Term	Description	Entries
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000409	Alanine	A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.	Abufène,Alanine, L-Isomer,L-Alanine,Alanine, L Isomer,L Alanine,L-Isomer Alanine
D053768	Metal Nanoparticles	Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.	Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic