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Suppression of Rickettsia typhi transmission in fleas maintained on murine typhus-immune rats. 1987

A F Azad, and M A Emala
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.

The presence of host antibodies against Rickettsia typhi in the hemolymph of Xenopsylla cheopis fed on immune rats was studied using an indirect immunofluorescent antibody (IFA) test and enzyme-linked immunosorbent assay (ELISA). The time course experiment revealed that antibody to R. typhi appears in the flea hemolymph after 18 hr exposure to host and persists for 24 hr. IFA tests utilizing specific antisera to rat IgG (Fc) and rat IgG (Fab) fragments, indicate that some unaltered rat IgG to R. typhi were present in the hemolymph of immune fed fleas. Host antibody to R. typhi was detected on the surface of the rickettsiae in the flea hemolymph and gut contents by IFA. Maintenance of infected fleas on immune host had no significant effect on the establishment and subsequent growth of rickettsiae within the fleas. However, 19-22 day infected fleas maintained on immune hosts failed to transmit R. typhi to baby rats.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008297 Male Males
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D005423 Siphonaptera An order of parasitic, blood-sucking, wingless INSECTS with the common name of fleas. Aphaniptera,Fleas,Flea
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D012286 Rickettsia typhi The etiologic agent of murine typhus (see TYPHUS, ENDEMIC FLEA-BORNE).
D014437 Typhus, Endemic Flea-Borne An infectious disease clinically similar to epidemic louse-borne typhus (TYPHUS, EPIDEMIC LOUSE-BORNE), but caused by RICKETTSIA TYPHI, which is transmitted from rat to man by the rat flea, XENOPSYLLA CHEOPIS. Murine Typhus,Typhus, Murine,Endemic Typhus,Murine Typhus Infection,Rickettsia typhi Infection,Endemic Flea-Borne Typhus,Flea-Borne Typhus, Endemic,Infection, Murine Typhus,Infection, Rickettsia typhi,Infections, Murine Typhus,Infections, Rickettsia typhi,Murine Typhus Infections,Rickettsia typhi Infections,Typhus Infection, Murine,Typhus Infections, Murine,Typhus, Endemic,Typhus, Endemic Flea Borne

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