-transparent.png){kind=logo}
The Bacillus subtilis bacteriophage phi 29 protein p6 binds to double-stranded DNA, but not to single-stranded DNA, as determined by a gel retardation assay. The nature of the interaction was further studied by DNase I "footprinting" experiments. Protein p6 binds to fragments containing the right or left terminal sequences of phi 29 DNA, producing a characteristic pattern of hypersensitive bands spaced about 24 nucleotides apart along most of the fragment, flanking protected regions. Binding of protein p6 to an internal phi 29 DNA fragment was also observed, but the footprint pattern was more salt sensitive than that obtained with the terminal phi 29 DNA fragments. By electron microscopy, protein p6 was shown to cover the DNA, totally or partially, from one end. In addition, binding of protein p6 to relaxed circular DNA induced positive supercoiling, indicating that a topological change in the DNA occurred.
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
October 1990,
Gene,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
November 1986,
Virology,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
June 1989,
Nucleic acids research,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
December 1989,
Nucleic acids research,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
December 1988,
Biochimica et biophysica acta,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
May 1982,
European journal of biochemistry,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
February 1991,
The Journal of biological chemistry,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
January 1989,
Journal of virology,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
January 2002,
Advances in virus research,
I Prieto, and
M Serrano, and
J M Lázaro, and
M Salas, and
J M Hermoso
January 1984,
Folia biologica,
