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The pepsin extraction of group A type 1 streptococci for the isolation of M protein fragments was studied at different pH values and at different time intervals. The extracts were compared by SDS PAGE and fused rocket immunoelectrophoresis. Type 1 M protein fragments were prepared in preparative scale by pepsin extraction of type 1 streptococci at pH 5.5 for 60 min. The fragments were separated by affinity chromatography on immobilized fibrinogen and finally purified for sequence studies by gel chromatography. Pepsin extraction of group A type 3 streptococci was also studied at different pH values. In contrast to type 1, the SDS PAGE pattern changed drastically in dependence on the pH. Affinity chromatography on immobilized fibrinogen is also effective in the separation of the pH 5.5 type 3 streptococcal pepsin extract.
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
November 1986,
FEBS letters,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
January 1976,
Allergie und Immunologie,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
June 2000,
Methods (San Diego, Calif.),
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
January 1989,
Infection and immunity,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
February 2006,
Molecular microbiology,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
July 1994,
Current microbiology,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
January 1996,
Advances in experimental medicine and biology,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
June 1987,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
April 2024,
bioRxiv : the preprint server for biology,
O Kühnemund, and
L Moravek, and
J Havlicek, and
W Köhler
June 1972,
The Journal of infectious diseases,
