OBJECTIVE Distortion-product otoacoustic emissions (DPOAEs) are currently used in many newborn hearing screening programs as the initial hearing test, typically testing frequencies between 1 and 4 or 6 kHz, but they have been associated with high false-positive rates. The objective was to investigate the possible benefit of high-frequency DPOAEs for reducing false-positive rates. METHODS 255 healthy newborns (138 males and 117 females) undergoing conventional hearing screening based on DPOAE and automated auditory brainstem response (AABR) testing were recruited. High-frequency DPOAE amplitudes, noise floors and signal-to-noise ratios (SNRs) were measured for f2 frequencies up to 12 kHz. RESULTS Of the 255 newborns who participated in this study, 23 (9%) failed the conventional DPOAE test but passed the AABR test, and 8 (3%) failed both tests. For an SNR threshold of 6 dB, high-frequency DPOAE tests at f2 = 4, 6, 8 and 10 kHz resulted in a reduction in the false-positive rate from 9% to 0.4%, or to zero if only three of the four frequencies were required to exceed the threshold. SNRs were lower in newborns with birth weights greater than 4000 g; lower at 2 kHz in newborns with a gestational age of 41 weeks; slightly higher in vaginally-delivered newborns; and higher at 2 kHz with increasing age in the group that failed the conventional DPOAE test but passed AABR. CONCLUSIONS High-frequency DPOAEs resulted in a reduction in the DPOAE failure rate and the false-positive rate. These findings may be helpful in universal newborn hearing screening programs.