-transparent.png){kind=logo}
Intact nuclear envelopes containing nuclear pore complexes have been prepared from the rat ventral prostate. The polypeptide profile of the nuclear envelopes from the rat prostate resembled that of nuclear envelopes prepared from the male rat liver. Isolation of the nuclear envelopes after incubation of purified nuclei with radioactive dihydrotestosterone results in labelling of the membrane. More dihydrotestosterone is bound after incubations at 22 degrees C for 18 h than at 2 degrees C for 18 h or 22 degrees C for 2 h. Scatchard analysis revealed a class of binding sites with an apparent Kd of 46 nM. Dihydrotestosterone, testosterone, cyproterone acetate and methyltrienolone were effective as competitors of labelled dihydrotestosterone binding to the nuclear envelopes, while estradiol did not compete. Castration of the rats 24, 48 and 96 h prior to preparation of nuclei resulted in loss of androgen binding to the membranes. Extraction with 0.6 M NaCl resulted in the loss of 72% of the androgen binding.
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
January 1971,
Biochimie,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
June 1982,
Endocrinology,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
December 1984,
Biochemical and biophysical research communications,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
August 1988,
Cell biology international reports,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
July 1978,
The Biochemical journal,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
June 1970,
The Biochemical journal,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
October 1983,
Biochimica et biophysica acta,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
September 1985,
Biochimica et biophysica acta,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
January 1986,
The Prostate,
Y A Lefebvre, and
E J Golsteyn, and
T L Michiel
January 1990,
Andrologia,
