BIOMAT Database Logo BIOMAT Database Logo

Protein kinase C activity and reactivity to phorbol ester in vascular smooth muscle from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). 1988

P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Department of Pharmacology, Sterling-Winthrop Research Institute, Rensselaer, New York 12144.

Protein kinase C (PKC) activity in aortic and renal arterial smooth muscle from SHR (20-23 wk male; mean arterial pressure = 178 mm Hg) and WKY (age/sex matched; mean arterial pressure = 126 mm Hg) was quantitated. Activity was greatest in the particulate fractions relative to the soluble fractions in all sources. The only difference between SHR and WKY was in the soluble fraction from SHR renal arteries, which had 2 fold more activity (255 pmol/mg/min) when compared with WKY (136 pmol/mg/min). This difference was not apparently related to force modulation, since the magnitude of isometric force development in renal arteries in response to phorbol 12,13-dibutyrate was not different between SHR and WKY. The magnitude of force developed in response to phorbol 12,13-dibutyrate and PKC activity in the particulate fraction was greatest in aorta vs. renal arteries in both WKY and SHR. These results suggest that regional vascular differences in the amount of PKC activity may exist which are not apparently related to a disease state (i.e., hypertension). These differences may be related to differential sensitivity to phorbol ester-mediated contractions in isolated smooth muscle.

UI MeSH Term Description Entries
D007537 Isometric Contraction Muscular contractions characterized by increase in tension without change in length. Contraction, Isometric,Contractions, Isometric,Isometric Contractions
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008297 Male Males
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D010703 Phorbol Esters Tumor-promoting compounds obtained from CROTON OIL (Croton tiglium). Some of these are used in cell biological experiments as activators of protein kinase C. Phorbol Diester,Phorbol Ester,Phorbol Diesters,Diester, Phorbol,Diesters, Phorbol,Ester, Phorbol,Esters, Phorbol
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D011918 Rats, Inbred SHR A strain of Rattus norvegicus with elevated blood pressure used as a model for studying hypertension and stroke. Rats, Spontaneously Hypertensive,Rats, SHR,Inbred SHR Rat,Inbred SHR Rats,Rat, Inbred SHR,Rat, SHR,Rat, Spontaneously Hypertensive,SHR Rat,SHR Rat, Inbred,SHR Rats,SHR Rats, Inbred,Spontaneously Hypertensive Rat,Spontaneously Hypertensive Rats
D011921 Rats, Inbred WKY A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR). Rats, Wistar Kyoto,Wistar Kyoto Rat,Rats, WKY,Inbred WKY Rat,Inbred WKY Rats,Kyoto Rat, Wistar,Rat, Inbred WKY,Rat, WKY,Rat, Wistar Kyoto,WKY Rat,WKY Rat, Inbred,WKY Rats,WKY Rats, Inbred,Wistar Kyoto Rats
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference

Related Publications

P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Protein kinase C activity in platelets from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY).
December 1986, Biochemical and biophysical research communications,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Vasodilator responses to cholinergic and adrenergic stimulants in spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) normotensive rats.
February 1983, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Vascular protein kinase C in Wistar-Kyoto and spontaneously hypertensive rats.
March 1992, European journal of pharmacology,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
A comparison of carotid body volumes in normotensive Wistar rats (NWR), Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR).
January 1987, Biomedica biochimica acta,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Early sucrose-induced retinal vascular lesions in spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY).
January 1986, Annals of clinical and laboratory science,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Adrenal dynorphin A and Leu-enkephalin levels in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR).
January 1990, Progress in clinical and biological research,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Analyzing the magnitude effect in spontaneously hypertensive (SHR) and wistar Kyoto (WKY) rats.
December 2020, Behavioural processes,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Different calcium storage pools in vascular smooth muscle cells from spontaneously hypertensive and normotensive Wistar-Kyoto rats.
May 1994, Journal of hypertension,
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Kidney extracts from spontaneously hypertensive rats (SHR) have greater dopamine 1A receptor RNA-binding activity than extracts from normotensive Wistar-Kyoto (WKY) rats.
October 1997, Clinical and experimental hypertension (New York, N.Y. : 1993),
P J Silver, and R E Lepore, and W R Cumiskey, and D Kiefer, and A L Harris
Difference in testosterone sensitivity in male spontaneously hypertensive (SHR) and Wistar-Kyoto rats (WKY).
September 1996, Physiology & behavior,
Copied contents to your clipboard!