Biomaterial Database

Urease from a sea urchin Lytechinus variegatus: partial purification and kinetics. 1979

R A Loest

Department of Biological Science, Florida State University, Tallahassee 32306.

1. Urease from a sea urchin Lytechinus variegatus, was purified 300-fold, using heat precipitation, ethanol precipitation and gel filtration. 2. The pH optimum is 8.0. 3. The apparent Michaelis constant for urea is 0.13 mM at pH 8.0. 4. The inhibitory effects of seven reagents on urease were evaluated. The pattern of inhibition is similar to other invertebrate ureases. 5. L. variegatus urease is compared with that of several other invertebrates, and its possible significance in CaCO3 formation is discussed.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012617	Sea Urchins	Somewhat flattened, globular echinoderms, having thin, brittle shells of calcareous plates. They are useful models for studying FERTILIZATION and EMBRYO DEVELOPMENT.	Echinoidea,Sand-Dollar,Clypeasteroida,Sand Dollars,Clypeasteroidas,Dollar, Sand,Dollars, Sand,Echinoideas,Sand Dollar,Sand-Dollars,Sea Urchin,Urchin, Sea,Urchins, Sea
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D014510	Urease	An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.	Phytourease,Urea Amidohydrolase,Amidohydrolase, Urea