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A competitive microtitre plate enzyme immunoassay for plasma aldosterone was developed using an immobilised aldosterone-bovine serum albumin conjugate and a monoclonal anti-aldosterone preparation, followed by the use of enzyme-labelled second antibodies to determine the degree of competition. The quantity of immobilised aldosterone-protein conjugate was adjusted to give optimum assay sensitivity with respect to the antibodies used. The lower limit of detection of aldosterone (55 fmol) was much less than that of an ELISA for aldosterone, using identical reagents but with an excess of immobilised aldosterone-protein conjugate, and up to 1400 fmol could be determined. Aldosterone levels in small amounts of male and female plasma could be assayed with good reproducibility.
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
March 1991,
The Journal of steroid biochemistry and molecular biology,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
February 1988,
Journal of steroid biochemistry,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
December 1978,
FEBS letters,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
September 1987,
Journal of immunological methods,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
April 1984,
The Journal of endocrinology,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
June 1987,
Journal of immunological methods,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
November 1985,
Clinical chemistry,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
March 1992,
Journal of immunological methods,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
December 1982,
Gan to kagaku ryoho. Cancer & chemotherapy,
C Hanquez, and
K M Rajkowski, and
B Desfosses, and
N Cittanova
February 1992,
Clinica chimica acta; international journal of clinical chemistry,
