-transparent.png){kind=logo}
Carcinoembryonic antigen (CEA) is important as one of the tumor markers, and enzyme-immunoassay using monoclonal anti-CEA, which is based on the Sandwich method and unnecessary for the treatment of CEA-extract from serum, was tried in this study. The standard curve obtained from this assay showed lineality on low CEA level. Sensitivity could allow to detect CEA below 5 ng/ml of CEA. Correlation between radioimmunoassay and this EIA was not found in especially high CEA-concentration. Reproducibility respect of Intra-assay and Inter-assay had good results. CEA value yielded by dilution of sample serum coincided with nearly expected value; this result indicated that dilution test was effective. CEA value determined by the assay to be necessary for treatment of extract was higher than that by the assay without treatment. Though the number of test samples used for assay of CEA in serum from various diseases were small, we considered it would be appropriate to set up a normal value range in 0.44-3.16 ng/ml (mean +/- 2 SD) and cut off value at 3.9 ng/ml (mean +/- 3 SD). We have been interested in cancer-specificity on monoclonal antibody, but we could not so for evaluate effects of the assay using monoclonal antibody.
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
July 1984,
Gan to kagaku ryoho. Cancer & chemotherapy,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
January 1987,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
March 1991,
The Journal of steroid biochemistry and molecular biology,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
August 1982,
Immunology letters,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
July 1974,
FEBS letters,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
November 1996,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
February 2002,
Hybridoma and hybridomics,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
September 2000,
Methods (San Diego, Calif.),
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
November 1996,
Journal of immunoassay,
K Arai, and
S Kanikawa, and
M Isomura, and
Y Takemori, and
Y Kanda, and
M Ishii
December 1988,
Journal of steroid biochemistry,
