-transparent.png){kind=logo}
The transport of newly synthesized cathepsin D in fibroblasts at 16-28 degrees C was compared to that at 37 degrees C. At 37 degrees C newly synthesized cathepsin D passes the trans Golgi within 30-60 min, becomes segregated from the secretory route into prelysosomal organelles within 1-2 h and processed to mature forms in dense lysosomes within 1.5-3 h after synthesis. The small fraction of cathepsin D that escapes transport into lysosomes is secreted within less than 2 h. At 16-28 degrees C the transport of cathepsin D to lysosomes is inhibited in a temperature-dependent manner. At 16-28 degrees C cathepsin D precursors are slowly transported to the trans Golgi. The cathepsin D precursors accumulate at a site that is in continuity with the secretory pathway and located within or distal of the trans Golgi and proximal to the site where cathepsin D precursors leave the secretory pathway as complexes with mannose 6-phosphate receptors. The arrest at this site is not complete. The receptor-dependent segregation of the cathepsin D precursors released from the block is impaired at less than or equal to 26 degrees C. The inhibition of segregation results in an increased, albeit retarded secretion of cathepsin D. The fraction of cathepsin D precursors that is segregated from the secretory pathway encounters a further low temperature block in prelysosomal organelles. There cathepsin D precursors are proteolytically processed to an intermediate form, which accumulates transiently.(ABSTRACT TRUNCATED AT 250 WORDS)
T Braulke, and
A Hasilik, and
K von Figura
July 1983,
The Journal of cell biology,
T Braulke, and
A Hasilik, and
K von Figura
January 1995,
Advances in experimental medicine and biology,
T Braulke, and
A Hasilik, and
K von Figura
November 1985,
European journal of biochemistry,
T Braulke, and
A Hasilik, and
K von Figura
January 1995,
Advances in experimental medicine and biology,
T Braulke, and
A Hasilik, and
K von Figura
January 1976,
Archives internationales de physiologie et de biochimie,
T Braulke, and
A Hasilik, and
K von Figura
January 1989,
Methods in cell biology,
T Braulke, and
A Hasilik, and
K von Figura
October 1992,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
T Braulke, and
A Hasilik, and
K von Figura
January 2008,
Neurobiology of aging,
T Braulke, and
A Hasilik, and
K von Figura
October 1992,
The Journal of biological chemistry,
T Braulke, and
A Hasilik, and
K von Figura
July 2002,
The American journal of pathology,
