BIOMAT Database Logo BIOMAT Database Logo

Allelic H3K27me3 to allelic DNA methylation switch maintains noncanonical imprinting in extraembryonic cells. 2019

Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115, USA.

Faithful maintenance of genomic imprinting is essential for mammalian development. While germline DNA methylation-dependent (canonical) imprinting is relatively stable during development, the recently found oocyte-derived H3K27me3-mediated noncanonical imprinting is mostly transient in early embryos, with some genes important for placental development maintaining imprinted expression in the extraembryonic lineage. How these noncanonical imprinted genes maintain their extraembryonic-specific imprinting is unknown. Here, we report that maintenance of noncanonical imprinting requires maternal allele-specific de novo DNA methylation [i.e., somatic differentially methylated regions (DMRs)] at implantation. The somatic DMRs are located at the gene promoters, with paternal allele-specific H3K4me3 established during preimplantation development. Genetic manipulation revealed that both maternal EED and zygotic DNMT3A/3B are required for establishing somatic DMRs and maintaining noncanonical imprinting. Thus, our study not only reveals the mechanism underlying noncanonical imprinting maintenance but also sheds light on how histone modifications in oocytes may shape somatic DMRs in postimplantation embryos.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D006657 Histones Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. Histone,Histone H1,Histone H1(s),Histone H2a,Histone H2b,Histone H3,Histone H3.3,Histone H4,Histone H5,Histone H7
D000483 Alleles Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product. Allelomorphs,Allele,Allelomorph
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015053 Zygote The fertilized OVUM resulting from the fusion of a male and a female gamete. Fertilized Ovum,Ovum, Fertilized,Fertilized Egg,Egg, Fertilized,Eggs, Fertilized,Fertilized Eggs,Zygotes
D016678 Genome The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA. Genomes
D047108 Embryonic Development Morphological and physiological development of EMBRYOS. Embryo Development,Embryogenesis,Postimplantation Embryo Development,Preimplantation Embryo Development,Embryonic Programming,Post-implantation Embryo Development,Postnidation Embryo Development,Postnidation Embryo Development, Animal,Pre-implantation Embryo Development,Prenidation Embryo Development, Animal,Development, Embryo,Development, Embryonic,Development, Postnidation Embryo,Embryo Development, Post-implantation,Embryo Development, Postimplantation,Embryo Development, Postnidation,Embryo Development, Pre-implantation,Embryo Development, Preimplantation,Embryonic Developments,Embryonic Programmings,Post implantation Embryo Development,Pre implantation Embryo Development
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018345 Mice, Knockout Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes. Knockout Mice,Mice, Knock-out,Mouse, Knockout,Knock-out Mice,Knockout Mouse,Mice, Knock out
D018392 Genomic Imprinting The variable phenotypic expression of a GENE depending on whether it is of paternal or maternal origin, which is a function of the DNA METHYLATION pattern. Imprinted regions are observed to be more methylated and less transcriptionally active. (Segen, Dictionary of Modern Medicine, 1992) Genetic Imprinting,Parental Imprinting,Imprinting, Genetic,Imprinting, Genomic,Imprinting, Parental

Related Publications

Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Maternal H3K27me3 controls DNA methylation-independent imprinting.
July 2017, Nature,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Genomic imprinting: mother maintains methylation marks.
July 2001, Current biology : CB,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
The conservation of allelic DNA methylation and its relationship with imprinting in maize.
February 2024, Journal of experimental botany,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages.
May 2018, Cell research,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
DNA methylation in genomic imprinting.
April 1997, Mutation research,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch.
September 2005, Molecular human reproduction,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem cells.
January 2013, PloS one,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
Genome-wide allelic methylation analysis reveals disease-specific susceptibility to multiple methylation defects in imprinting syndromes.
April 2013, Human mutation,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
DNA methylation, imprinting and cancer.
January 2002, European journal of human genetics : EJHG,
Zhiyuan Chen, and Qiangzong Yin, and Azusa Inoue, and Chunxia Zhang, and Yi Zhang
DNA methylation and genomic imprinting.
May 1994, Cell,
Copied contents to your clipboard!