-transparent.png){kind=logo}
Selected synthetic luteinizing hormone releasing hormone preparations were assayed, and their potencies were determined relative to one sample utilizing primary cultures of enzymatically dispersed rat anterior pituitary cells. Preliminary cell culture experiments indicated that luteinizing hormone releasing hormone had to be in constant contact with cells for continued luteinizing hormone secretion. Luteinizing hormone levels in media reached a maximum concentration after 4 hr of continuous luteinizing hormone releasing hormone exposure. Cell culture bioassay was selected over the bioassay employing chronically ovariectomized steroid-blocked rats due to greater sensitivity and economy. The assay of each luteinizing hormone releasing hormone preparation was replicated four to seven times. Preparations from several companies were less potent (p less than 0.05) than the reference product. Contaminants were disclosed by TLC in preparations with potencies lower than the reference product.
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
July 1981,
Endocrinology,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
March 1991,
Biochimica et biophysica acta,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
October 1981,
Endocrinology,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
September 1980,
Proceedings of the National Academy of Sciences of the United States of America,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
February 1986,
Molecular and cellular endocrinology,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
January 1982,
Acta endocrinologica,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
September 1985,
Zentralblatt fur Veterinarmedizin. Reihe A,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
February 1986,
American journal of obstetrics and gynecology,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
April 1994,
Histology and histopathology,
W C Dermody, and
C A Pastushok, and
R Sakowski, and
J W Vaitkus, and
J R Reel
February 1975,
Endokrinologie,
