A scheme for identification of nonfermentative gram-negative bacteria. 1977

M Shayegani, and A M Lee, and L M Parsons

A scheme was designed to speed the identification of 50 nonfermentative gram-negative bacteria to genus, species, and biotype using a minimal numer of biochemical tests. When the scheme was evaluated in our laboratory, one technologist identified 93.6% of 188 known isolates (46 species). Later another technologist, given 161 of the same isolates (46 species) as unknowns, identified 91.9%. The isolates that were misidentified in both cases were atypical organisms with aberrant biochemical reactions, plus 1 typical organism with a delayed oxidation of glucose.

UI MeSH Term Description Entries
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D006088 Gram-Negative Aerobic Bacteria A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine. Achromatiaceae,Achromatium,Achromobacteriaceae,Gram Negative Aerobic Bacteria
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

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