-transparent.png){kind=logo}
Chicken gizzard muscle caldesmon has been examined for ability to interact with tropomyosin from chicken gizzard muscle by using fluorescence enhancement of tropomyosin labeled with dansyl chloride (DNS) and affinity chromatography. The binding of caldesmon to tropomyosin was regulated by Ca2+ and calmodulin, i.e., at low ionic strength most of the caldesmon bound to tropomyosin-Sepharose 4B was co-eluted by adding calmodulin only in the presence of Ca2+, but not in its absence. This regulation by Ca2+ and calmodulin was also suggested by fluorescence measurements. Actin- and calmodulin-binding sites on the caldesmon molecule were located in the 38K fragment (Fujii, T., Imai, M., Rosenfeld, G.C., & Bryan, J. (1987) J. Biol. Chem. 262, 2757-2763). When 38K-enriched fraction was applied to the tropomyosin-Sepharose, the 38K fragment was retained by the column and could be eluted by adding Ca2+ and calmodulin.
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
February 1987,
The Journal of biological chemistry,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
June 1993,
Biological & pharmaceutical bulletin,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
April 1996,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
September 1985,
The Biochemical journal,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
June 1987,
FEBS letters,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
November 1988,
The Biochemical journal,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
February 1998,
Tissue & cell,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
June 1984,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
October 1988,
Biochemical and biophysical research communications,
T Fujii, and
J Ozawa, and
Y Ogoma, and
Y Kondo
June 1985,
The Journal of biological chemistry,
